Skip to main content
. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: Oncogene. 2013 Dec 23;34(3):334–345. doi: 10.1038/onc.2013.552

Figure 2.

Figure 2

Oncogenic activation and autophagy induction in Tax-GFP-established T cell lines. (a) Immunophenotype of Tax-GFP immortalized T cell lines, PTX4-1 and PL9-1, as determined by FACS analysis. (b) EMSA assay to detect the activities of NF-κB, Stat3, AP-1, NF-ATc and OCT-1 in Tax-established cells. Human primary CD4+ T cells, which were used as control, were isolated from healthy donor using anti-CD4 conjugated magnetic beads, and stimulated with PHA (1μg/ml) for 1 day, followed by adding IL-2 (100u/ml, every other day) for 2 weeks. (c) Anti-LC3 immunoblot to examine the conversion of LC3-I to LC3-II and Tax expression in Tax-GFP-established T cell lines, PTX4-1 and PL9-1.