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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Mol Immunol. 2010 Nov 18;48(4):431–441. doi: 10.1016/j.molimm.2010.09.016

Fig. 1. Physical–chemical characterization of rBet v 1 wildtype and rBet v 1 trimer by SDS-PAGE.

Fig. 1

(A) Comparison of purified recombinant Bet v 1 and rBet v 1 trimer (left). rBet v 1 trimer run in the presence (+Met) or absence (−Met) of 2-mercaptoethanol. M, molecular mass markers (kDa). (B) Mass spectrometric analysis of rBet v 1 and rBet v 1 trimer. The mass/charge ratios are shown in the x-axes, and the signal intensities are displayed in the y-axis as absolute value in the investigated mass range. (C) Circular dichroism analysis of rBet v 1 and rBet v 1 trimer. The mean residue ellipticities (y-axes) are shown for rBet v 1 trimer and for rBet v 1 at different wavelengths (x-axes).