Figure 7.

Bcl-xL and XIAP participate in GSK-3i-induced sensitization to TRAIL/ and TNFα. (a) Representative flow cytometric profile of annexin V-FITC and PI stained HupT3 cells to evaluate apoptosis induction. HupT3 cells with stable expression of lentiviral shRNA for Bcl-xL, cIAP2 or XIAP (or scrambled control) were treated with diluent, TNFα or TRAIL as indicated for 18 h before harvesting and staining. (b) Summarized results from three independent analyses as percentage of annexin V-FITC and/or PI cells to total gated cells were shown as average±S.D. (c, d) Whole-cell extracts from above HupT3 cells (c) and BxPC-3 cells stably re-expressing Flag.Bcl-xL (d) were subjected to immunoblotting to confirm efficient suppression of endogenous gene expression and rescued Bcl-xL expression. (e) BxPC-3 cells with stable lentiviral-mediated expression of Flag.Bcl-xL were treated and stained as in (a) for apoptosis analysis