Table 2.

Transcription of anti-sense strand of KLF16 in CMV–CMV vector

Vector	RT primer	copy number/ng RNA (×103)
CMV–CMV	KLF16/SS	0.29 ± 0.01
CMV–KLF16–CMV		51.48 ± 7.48
CMV–CMV (flipped)		0.38 ± 0.03
CMV–KLF16–CMV (flipped)		48.72 ± 5.35

After transfection, copy number of anti-sense strand of KLF16 mRNA was measured. Data are mean ± SD