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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Protein Expr Purif. 2010 Dec 10;76(2):254–263. doi: 10.1016/j.pep.2010.12.002

Figure Eight.

Figure Eight

Production of “all-Q” mutant. The sample of “all-Q” mutant of rFH (left-hand gel) migrates as a single band during SDS–PAGE under reducing (R) and non-reducing (NR) conditions (stained by Coomassie blue). Endo Hf (77 kDa) treatment causes no change in migration rate. This is consistent with the “all-Q” mutant having no N-glycosylation sites and being glycan-free. For comparison, rFH (prior to purification) migrates as a fuzzy band until it is Endo Hf treated (right-hand gel). MW = molecular weight markers as indicated to left and right of the two gels.