Table 1.

Primers used for species-specific EHS PCR assays

Species	Forward primer	Reverse primer	Reference
H. hepaticus	5′ GCAUUUGAAACUGUUACUCUG 3′	5′ GGGGAGCUUGAAAACAG 3′	34
H. bilis	5′ AGAACTGCATTTGAAACTACTTT 3′	5′ GGTATTGCATCTCTTTGTATGT 3′	14
H. typhlonius	5′ AGGGACTCTTAAATATGCTCCTAGAGT 3′	5′ ATTCATCGTGTTTGAATGCGTCAA 3′	10
H. rodentium	5‘ GTCTT AGT TGC TAA CTA TT 3′	5′ AGA T'IT GCT CCA TTT CAC AA 3′	36
H. rodentium*	5′ GTGGAGTGCTAGCTTGCTAGAA 3′	5′ ACCGTAGCATAGCTGATCTA 3′	current study
H. apodemus	5′ TGGGAGTGCCCTTTTAGGGAG 3′	5′ TGAGATTTGCTCCATTTCAC 3′	current study
H. mastomyrinus	5′ AGAACTGCATTTGAAACTATGAG 3′	5′ CAGTATTGCGTCTCTTTGTA 3′	35

The H. apodemus-specific primers and H. rodentium* primers were designed for the current study and used to distinguish H. rodentium from H. apodemus.