Skip to main content
. 2014 Jun 19;21(6):732–742. doi: 10.1016/j.chembiol.2014.03.014

Figure 1.

Figure 1

Amyloid Fibril Formation and Fluorescence Lifetimes of the HF488-Labeled Aβ Peptides In Vitro

(A) Amyloid formation by HF488Aβ(1–42) (5 μM in 50 mM sodium phosphate buffer [pH 7.4]) as a function of time. The quantity of amyloid fibrils was monitored by immunochemistry using the conformation-specific antibody LOC (red circles and dashed line) to detect fibrillar species. The normalized fibril quantity (relative to the maximum value) is given on the right axis in the graph. The amyloid formation was also monitored by the decrease in fluorescence emission intensity of the HF488 dye (blue). a.u., arbitrary units. See also Figure S1A.

(B) Far UV circular dichroism spectra of monomeric and fibrillar Aβ(1–42).

(C) Fluorescence lifetime images of solutions of monomeric and fibrillar HF488Aβ(1–42). The lifetime color coding is shown in the bar to the right of the images. The frequency histograms show the per-pixel distribution of HF488 fluorescence lifetimes in each of the images.

(D) Mean fluorescence lifetime (± SD) of the unconjugated HF488 dye at different pH values and for monomeric, fibrillar, and trypsin-treated monomeric HF488Aβ(1–40).