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Light sources and filters used for wide field fluorescence imaging
| Fluorophore | Light source & channel | Filter cube | ||
|---|---|---|---|---|
| Excitation | Beamsplitter | Emission | ||
| GFP | Colibri/LED470* Spectra-6/C§ | FF01-475/28† LL01-488/1† | T495LP‡ | FF01-525/50† |
| tdEos (red emission) | Colibri/LED555* Spectra-6/GY§ | BP550/25* FF01-543/3† | FT570* FF568-Di01† | BP605/70* FF01-593/46† |
| tdEos (photoconversion) | Colibri/LED405* Spectra-6/V§ | FF01-405/10† | 59004BS‡ | 59004M‡ |
Source: *Zeiss.
Semrock.
Chroma.
Lumencor.
