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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Mech Dev. 2004 Nov;121(11):1393–1401. doi: 10.1016/j.mod.2004.06.001

Fig. 1.

Fig. 1

Changes in cellular organization in turtle gonad development. Male or female turtle gonads were collected from the temperature-sensitive stages (stages 16–19, only stage 17 shown as a representative sample), immediately after the temperature-sensitive stages (stages 20–21, only stage 20 is shown as a representative sample), and close to hatching (stage 22—hatching, only stage 23 is shown as a representative sample). Cellular organization was visualized by immunostaining for laminin (green) and VASA (red). Laminin outlined the basal membrane of primitive sex cords (green) and VASA marked the primordial germ cells (red). Arrows indicate the connections of primitive sex cords to the cortical region of the gonads. The temperature sensitive period is between stage 17 and 20.