FIGURE 1.

Illustration of the coculture system in the transwell. (A) Coculture of GEnCs and podocytes with or without being in juxtaposition. Podocytes and GEnCs on the opposite side of the porous insert formed cocultures in juxtaposition. Cocultures without being in juxtaposition were formed allowing the cell types to communicate through release of soluble mediators. Neutrophil recruitment was assessed to represent all cells that had bound to the GEnCs (i.e., firmly adherent and transmigrated). (B) Fluorescent micrographs showing neutrophil binding to and transmigration through GEnC/podocyte cocultures: (i) Apical surface of transwell insert with neutrophils adherent to GEnCs in coculture, (ii) basal side of transwell insert with transmigrated neutrophils adjacent to podocytes in coculture, (iii) apical surface of transwell insert with neutrophils adherent to GEnCs in monoculture, and (iv) basal side of transwell insert with transmigrated neutrophils in GEnC monocultures. Original magnification ×40.