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Detection of CXCL1, CXCL5, and CXCL8 in cocultures and monocultures. Detection of soluble (A) CXCL1, (B) CXCL5, and (C) CXCL8 in supernatants collected from TNF-α–stimulated cocultures (●), GEnC monocultures (▪), and podocyte monocultures (▴). Data expressed as means ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.0001 comparing the difference in chemokine expression between cocultures and GEnC monocultures by two-way ANOVA.
