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Neutralization of CXCL1, CXCL5, and CXCL8 in cocultures and monocultures. Cultures were treated with function neutralizing Abs against (A) CXCL1 (2 μg/ml), (B) CXCL5 (5 μg/ml), and (C) CXCL8 (10 μg/ml) for 24 h prior to addition of neutrophils. Data are means ± SEM (n = 5–6). *p < 0.05, **p < 0.01, ***p < 0.0001 comparing the effect of blocking chemokine function in cultures with their controls by a paired t test.
