(A) GT-tg female mice were ovariectomized. After one week of recovery, mice (n = 12 – 16 / group) were intraperitoneally treated with 7 days of saline (as a control; open bars) or doxycycline (to centrally induce HIV-1 Tat protein; striped bars) while subcutaneously co-administered vehicle, estradiol (0.09 mg/kg), and/or progesterone (4 mg/kg). Anxiety-like behavior was assessed in (B) an open field, (C) an elevated plus maze, and (D) a marble burying task. * indicates significant interaction wherein indicated doxycycline-treated group differs from respective saline-treated control group. † indicates significant main effect for estradiol-administered mice to differ from vehicle-treated mice. ‡ indicates significant main effect for progesterone-administered mice to differ from estradiol- or estradiol/progesterone-administered mice. § indicates significant interaction wherein doxycycline/progesterone-treated group differs from doxycycline/vehicle- or doxycycline-estradiol-treated groups, p < 0.05.