Table 2.

Ovariectomized GT-tg mice were treated i.p. for 7 days with saline as a control, or doxycycline (100 mg/kg) to induce HIV-1 Tat protein, concurrent with administration of s.c. vehicle, estradiol (0.09 mg/kg), and/or progesterone (4 mg/kg) and were assessed for motor behavior (movement velocity, total arm entries) and anxiety-like behavior (latency to enter the brightly-lit center, % time spent on brightly-lit open arms) in an open field and elevated plus, respectively.

		Vehicle		Estradiol		Progesterone		Estradiol/ Progesterone
		Saline (n=14)	Doxycycline (n=15)	Saline (n=15)	Doxycyclin (n=16)	Saline (n=15)	Doxycycline (n=16)	Saline (n=16)	Doxycycline (n=12)
Motor Measures
Movement Velocity (cm / s ±SEM)		6.8 ±0.3	7.2 ± 0.3	6.4 ±0.3†	6.0 ± 0.4†	6.6 ±0.2	6.8 ± 0.3	6.3 ±0.3†	6.3 ± 0.3†
Total Arm Entries (mean±SEM)		18 ±1	17 ± 1	19 ±1	14 ± 2	19 ±1	17 ± 1	15 ±1	16 ± 2
Anxiety-like Measures
Latency to Central Entry (s±SEM)		88 ±19	75 ± 21	55 ±16	73 ± 13	28 ±6†‡	42 ± 12†‡	104 ±24	62 ± 16
% Open Arm Time (% ± SEM)		27 ±4	20 ± 2	33 ±5	19 ± 3^	30 ±5	33 ± 5§	22 ±4	29 ± 5

A main effect of hormone treatment indicates groups significantly differ from vehicle-treated mice (†) or estradiol/progesterone-treated mice (‡), irrespective of doxycycline condition.

An interaction between hormone and doxycycline conditions indicates that estradiol/doxycycline-treated mice significantly differ from their estradiol/saline-treated counterparts (^) and progesterone/doxycycline-treated mice significantly differ from estradiol/doxycycline-treated mice or vehicle/doxycycline-treated controls (§), p < 0.05.