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Effect of 4-AP on RPMI-8226 cell viability and cell proliferation. (a) Different concentrations of 4-AP ranging from 0.5 mM to 8 mM were added to examine viability in 24 h, 48 h, 72 h, and 96 h. Data were plotted as means ± SD. (b) Cells were divided and cultured in the normal medium, serum-free medium, and 2 mM 4-AP containing medium for 48 hours. (c) Effects of 4-AP in various concentrations on RPMI-8226 cells proliferation.
