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. 2014 Jun 23;205(6):829–846. doi: 10.1083/jcb.201403021

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© 2014 Taylor et al.

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Figure 3. — Mapping of the Ca_Vβ_1a nuclear localization domain. (A) Diagram of constructed Ca_Vβ_1a-YFP truncation mutants and respective cytoplasmic and nuclear intensity in untreated and LMB-treated cells. Conserved SH3 and GK domains are noted in dark blue, putative NLS highlighted in purple, and YFP sequence in green. Construct names indicate amino acids remaining after truncation, with Ca_Vβ_1a-1-524 as full-length Ca_Vβ_1a. Table reflects relative intensity of cytoplasmic (Cyto) and nuclear Ca_vβ_1a. (B) Enlarged image of Ca_Vβ_1a-161-524, which is absent from the nucleus after LMB treatment. (C) Enlarged image of Ca_Vβ_1a-101-274, which is present in the nucleus without LMB treatment. Nuclei (DNA) in all images were stained blue with Hoechst dye. Bars: (B and C) 50 µm.