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. 2014 Jun 23;205(6):829–846. doi: 10.1083/jcb.201403021

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© 2014 Taylor et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Regulation of myoblast proliferation by Ca_vβ_1a in vitro and in vivo. (A) Quantification of myoblast growth for 7 d after transfection with either scrambled control shRNA or Ca_vβ_1a-targeted shRNA (Western blot of Ca_vβ_1a knockdown is inset). (B) Quantification of MPCs cultured from Cacnb1^+/− and Cacnb1^−/− embryos for 4 d (n = 4). (C–E) Primary mouse myoblasts transfected with EGFP (C) or Ca_vβ_1a-YFP (D) and stained 24 h later for Ki67 (red; n = 3). Bar, 100 µm. (E) Quantification of Ki67+/EGFP and Ki67+/Ca_vβ_1a-YFP cells expressed as a percentage of total EGFP or Ca_vβ_1a-YFP + cells. *, P < 0.05.