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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Chem Biol Drug Des. 2014 Apr;83(4):440–449. doi: 10.1111/cbdd.12259

Figure 2.

Figure 2

Bioassay-guided fractionation and identification of an active compound. (A) Using fluorescent puncta in yeast expressing Q103-GFP as a guide, the active HPLC fractions in crude extracts of SID#13569 were determined. Representative images are shown, and the arrows indicate puncta. Scale bar is 2 μm. The results were quantified by counting more than 200 yeast from random fields and scoring individual cells for the presence of well-resolved puncta. (B) The chemical structure of actinomycin D.