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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Chem Biol Drug Des. 2014 Apr;83(4):440–449. doi: 10.1111/cbdd.12259

Figure 3.

Figure 3

Confirmation of actinomycin D as an active compound. (A) Authentic actinomycin D (Sigma #A12110) improved growth of yeast expressing ΔProQ103. Results are representative of experiments performed in triplicate. Uninduced cells were grown in 4% raffinose. (B) Actinomycin D blocked the formation of puncta in cells expressing Q103-GFP. Representative images are shown, with puncta indicated by white arrows. Scale bar is 2 μm. For quantifications, more than 200 yeast cells were examined and scored for the presence of puncta.