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Suppression of polyQ aggregation in mammalian PC12 cells. (A) In PC12 cells, aggregation of htt103Q-EGFP into puncta is observed at 48 h after induction. Low concentrations of actinomycin D (4–100 ng/mL) reduced the number of cells with aggregates. Scale bar = 50 μm. (B) Quantification of the percentage of PC12 cells with aggregates averaged from three independent experiments ±SEM.
