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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Free Radic Res. 2014 Feb 24;48(4):487–502. doi: 10.3109/10715762.2014.886775

Figure 6.

Figure 6

Characterization of the integrated time data from rat liver cytosolic GPx-1 for Forstrom et al. [36] for different hydroperoxides. Here, the enzyme assays were carried out at 0.15 M ionic strength and 37°C at pH 7.2 in Tris-HCl buffer. (A) Removal and generation of CumOOH (16 μM) and the product [GSSG], respectively, by rat liver GPx with time for three different [GSH] of 0.13 mM (solid line), 0.21 mM (dashed line), and 0.54 mM (dotted line). (B) Removal and generation of LinOOH (16 μM) and the product [GSSG], respectively, by rat liver GPx with time for three different [GSH] of 0.17 mM (solid line), 0.27 mM (dashed line), and 0.54 mM (dotted line). (C) Model description of the integrated time data for CumOOH (A0) for different [GSH] of 0.13, 0.17, 0.21, 0.27, and 0.54 mM. (D) Model description of the integrated time data for LinOOH (A0) for different [GSH] of 0.17, 0.21, 0.27, 0.4, 0.54, and 0.81 mM. Pt represents product [GSSG]. E0 is the concentration of enzyme used 5.2 nM.