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. Author manuscript; available in PMC: 2014 Aug 1.

Published in final edited form as: Curr Opin Chem Biol. 2013 Jul 24;17(4):571–579. doi: 10.1016/j.cbpa.2013.06.020

Working models accounting for the IspH-catalyzed reaction. (A) Birch reduction model. In this model, the iron-sulfur cluster has two functions: (1) mediating two sequential one-electron reduction, and (2) serving as the Lewis acid to facilitate C₄-dehydroxylation. (B) Organometallic model. This model has two unique features: (1) the HMBPP C₄-OH group rotates away from the [4Fe-4S] cluster to form an internal H-bond (40), and (2) an iron-sulfur cluster mediated two-electron reductive dehydroxylation step (40 → 41). (C) Substrate bound active site structure of wt and E126Q variant IspH.

Working models accounting for the IspH-catalyzed reaction. (A) Birch reduction model. In this model, the iron-sulfur cluster has two functions: (1) mediating two sequential one-electron reduction, and (2) serving as the Lewis acid to facilitate C₄-dehydroxylation. (B) Organometallic model. This model has two unique features: (1) the HMBPP C₄-OH group rotates away from the [4Fe-4S] cluster to form an internal H-bond (40), and (2) an iron-sulfur cluster mediated two-electron reductive dehydroxylation step (40 → 41). (C) Substrate bound active site structure of wt and E126Q variant IspH.