Chromosome organization in E.
coli BW25113 ΔtolC cells treated with 1 and 3. (a, c) Treatment of cells with both
compounds produced filamentous
cells. (b, d) DAPI labeled cells demonstrate that treatment with 3 produced compact chromosomes, whereas treatment with 1, known to induce dsDNA breaks, produced diffuse chromosomes.
This experiment indicates differences in the mechanism of DNA gyrase
inhibition by these two compounds.