FIG 2 .

Mpl-region RdRp phylotypes. An unrooted maximum-likelihood (ML) tree based on an alignment of the translated RdRp region targeted by the degenerate Mpl primer sets described by Culley and Steward (18) is shown. Included in this analysis are environmental sequences (black branches) that are comprised of sequences from this study, as well as from three previous studies published by Culley and Steward (17, 18, 25), and homologous sequences from representative viruses from the established taxa (orange branches) in the order Picornavirales. The specific sequences in the alignment are listed in Table S1 in the supplemental material. The scale bar is the equivalent of 0.3 substitutions per site. The statistical support values shown are percentages calculated by the aLRT method. Dashed branches represent clades with support values greater than 80. CcloRNAV, Cylindrotheca closterium RNA virus 01 and 02.