Fig. 8.
Aβ1–40 oligomers in transgenic C. elegans strains recognized by SPR-based immunoassay. Lysates from transgenic worms expressing Aβ1–40WT (WT) or Aβ1–40A2V (A2V) were flowed for 3 min (bar) onto a sensor chip on which we previously immobilized 4G8. Lysates from worms transfected with the empty vector were used as control (Vector). The three-min association phase was followed by 11 min of dissociation. The figure shown the obtained sensorgrams, i.e. the time course of the SPR signal expressed in resonance units (RU), obtained in a single experimental session. Very similar results were found in four independent sessions, with new sensor-chips and ex novo preparations of worm lysate.