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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: J Immunol. 2013 Apr 29;190(11):5779–5787. doi: 10.4049/jimmunol.1200293

Figure 1.

Figure 1

Nematode infection induced up-regulation of SerpinB2 expression in the small intestine dependent on STAT6. C57BL/6 mice (WT) mice were infected with H. bakeri (Hb) or N. brasiliensis (Nb) third stage larvae. (A) The kinetics of SerpinB2 mRNA expression in the small intestine of mice at days 4, 7, 10, and 14 post Hb infection (DPI) was analyzed by qPCR. The fold changes were relative to the vehicle (VEH) group after normalization to 18S rRNA. (B) The intestinal lysates (60µg) were analyzed on 4–12% Bis-Tris NuPage gels and probed with anti-mouse SerpinB2. The 46-kDa SerpinB2 protein and a cleavage product of SerpinB2 with lower molecular weight (~37 kDa) were detected (arrows). Negative controls: identically prepared intestinal lysates from SerpinB2−/− mice. Positive controls: LPS-treated RAW 264.7 murine macrophages (12µg) and bone marrow-derived macrophages (BMDM, 40 µg). Immunoblot was reprobed with anti-GAPDH as a loading control. n.s. indicates nonspecific immunoreactive bands as evidenced by their presence in intestinal lysates from SerpinB2−/− mice. (C) qPCR analysis of the SerpinB2 expression in intestines from WT and STAT6−/− mice at day 10 post Nb infection. *p<0.05 versus VEH (n≥5 for each group).