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. Author manuscript; available in PMC: 2015 May 30.
Published in final edited form as: Biochem Biophys Res Commun. 2014 Apr 20;448(2):134–138. doi: 10.1016/j.bbrc.2014.04.063

Figure 1.

Figure 1

Gleevec blocks the phosphorylation of p190RhoGAP by SEMA3A and SEMA3F. U87MG cells were pre-treated with Gleevec (100 μM) 1 hour prior to SEMA3A and SEMA3F (320 ng/ml) treatment. After 10 minutes, cells were lysed and immunoprecipitated with anti-p190RhoGAP antibody. Western blotting analysis was carried out with anti-phosphotyrosine antibody or anti-p190RhoGAP antibody. The band intensity of phosphorylated p190RhoGAP bands was normalized to their respective total p190RhoGAP levels. The bar graph represents the fold-change in band intensity relative to control.