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HPLC elution profile of pool RNA that has been treated with nuclease P1 and alkaline phosphatase as described in Basic Protocol 2. (A) Unmodified pool, (B) pool containing 5-hydroxymethyluridine, (C) pool containing 5-imidazolemethyluridine, (D) pool containing 5-phenolmethyluridine (Robertson, 2001).
