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Serial sections of a lymph node from a pig with experimental postweaning multisystemic wasting syndrome. Porcine circovirus type 2b (PCV2b) antigens were detected in 16 macrophages per 0.25 mm2 with polyclonal-antibody-based immunohistochemical (IHC) testing (A) and in 5 macrophages per 0.25 mm2 with monoclonal-antibody-based IHC testing (B). With PCV2b-based in-situ hybridization, PCV2b DNA was detected in 25 macrophages per 0.25 mm2 (C).
