TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| E. coli pMOS Blue | F′ endA1 hsdR17 (rK− mK+), supE44 thi-1 recA1 gyrA96 relA1 lac [F′ lacIqZΔM15 proAB+ Tn10 (Tetr) | GE Healthcare |
| M. tuberculosis H37Rv ATCC 27294 | Virulent laboratory strain of M. tuberculosis | AZI collection |
| M. bovis BCG—CoaA OE strain | MtPanK-overexpressing M. bovis BCG | This study |
| Plasmids | ||
| pBAN0477 | M. tuberculosis coaA gene cloned at NdeI and BamHI cloning site, Hygr | This study |
| pAZI9018b | E. coli-mycobacterial IPTG-inducible shuttle vector—a replicating vector with lacZ, Hygr, NdeI, and BamHI as cloning sites | 12 |
| pBAN0317 | M. tuberculosis coaA conditional expression vector with truncated, mutant coaA gene cloned downstream of pristinamycin-inducible promoter (pPTR) | This study |
| pAZI9479 | Mycobacterial conditional-expression (integrating) vector with pPTR system | 13 |
a
Tetr, tetracycline resistance; Hyg, hygromycin resistance.