TABLE 3.
MICs for 40 M. genitalium strains with isolation histories and presence of mutations
| Strain ID (reference)a | Sample collection yr | Country | MIC (μg/ml)b |
Mutationc |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| AZM | ERY | SOL | CIP | MXF | DOX | 23S rRNA | rpl4 | rpl22 | |||
| G37 (1) | 1980 | United Kingdom | 0.008 | 0.125 | 0.002 | 8 | 0.125 | 0.5 | WT | WT | WT |
| M30 (1, 29) | 1980 | United Kingdom | 0.008 | 0.125 | ≤0.001 | 8 | 0.125 | 0.5 | WT | WT | WT |
| M2288 (46) | 1991 | Denmark | 0.002 | 0.125 | ≤0.001 | 0.5 | 0.125 | 0.063 | WT | N172S, A114V, A116V | WT |
| M2282 (46) | 1991 | Denmark | 0.004 | 0.063 | ≤0.001 | 4 | 0.125 | 0.25 | WT | WT | WT |
| M2300 (46) | 1991 | Denmark | 0.008 | 0.125 | ≤0.001 | 16 | 0.125 | 0.25 | WT | WT | WT |
| M2321 (46) | 1991 | Denmark | 0.004 | 0.063 | ≤0.001 | 8 | 0.25 | 0.5 | WT | WT | WT |
| M2341 (46) | 1991 | Denmark | 0.008 | 0.25 | ≤0.001 | 2 | 0.25 | 1 | WT | WT | WT |
| M6090d (29) | 1994 | France | 0.004 | 0.063 | ≤0.001 | 2 | 0.063 | 0.125 | WT | WT | WT |
| M6151d (29) | 1994 | France | 0.008 | 0.125 | ≤0.001 | 4 | 0.125 | 0.25 | WT | WT | WT |
| M6312d (30) | 1994 | France | 0.004 | 0.063 | ≤0.001 | 8 | 0.125 | 0.25 | WT | WT | WT |
| M6285 (47) | 1997 | Sweden | 0.004 | 0.125 | ≤0.001 | 1 | 0.125 | 0.5 | WT | WT | WT |
| M6280 (47) | 1997 | Sweden | 0.004 | 0.063 | ≤0.001 | 1 | 0.063 | 0.125 | WT | N172S | WT |
| M6328 (47) | 1998 | Sweden | 0.004 | 0.125 | ≤0.001 | 2 | 0.125 | 0.25 | WT | N21K | A43V |
| M6281 (47) | 2001 | Sweden | 0.008 | 0.063 | ≤0.001 | 0.5 | 0.125 | 0.25 | WT | WT | WT |
| M6286 (47) | 2001 | Sweden | 0.002 | 0.03 | ≤0.001 | 2 | 0.03 | 0.25 | WT | A114S, R45K | WT |
| M6287 (47) | 2003 | Japan | 0.002 | 0.03 | ≤0.001 | 4 | 2 | 0.125 | WT | P81S | WT |
| M6284 (47) | 2003 | Japan | 0.004 | 0.125 | ≤0.001 | 4 | 0.125 | 0.25 | WT | WT | WT |
| M6283 (47) | 2003 | Japan | 0.004 | 0.063 | ≤0.001 | 2 | 0.125 | 0.5 | WT | WT | WT |
| M6282 (47) | 2003 | Japan | 0.008 | 0.125 | ≤0.001 | 1 | 0.125 | 0.25 | WT | WT | WT |
| M6311 | 2004 | Sweden | 0.004 | 0.125 | ≤0.001 | 1 | 0.063 | 0.5 | WT | WT | WT |
| M6327 (22) | 2005 | Denmark | 0.016 | 0.125 | 0.002 | 8 | 0.25 | 0.25 | WT | WT | WT |
| M6375 | 2005 | Sweden | 0.004 | 0.063 | ≤0.001 | 1 | 0.03 | 0.125 | WT | WT | WT |
| M6315 | 2005 | Sweden | 0.002 | 0.063 | ≤0.001 | 1 | 0.125 | 0.5 | WT | WT | WT |
| M6475 | 2006 | Sweden | 0.008 | 0.125 | ≤0.001 | 1 | 0.5 | 0.5 | WT | WT | WT |
| M6713 | 2010 | Australia | 0.063 | 0.03 | ≤0.001 | 0.5 | 0.125 | 0.5 | WT | P81S | WT |
| M6303 (22) | 2003 | Norway | >8 | >16 | 0.5 | 8 | 0.25 | 2 | A2059G | H69R | WT |
| M6270 (22) | 2004 | Australia | >8 | >16 | 0.5 | 4 | 0.125 | 0.25 | A2059G | WT | E123K |
| M6320 (22) | 2004 | Australia | ≥64 | ≥64 | 0.25 | 0.5 | 0.063 | 0.25 | A2059G | WT | WT |
| M6489 | 2007 | Sweden | >16 | >16 | 1 | >16 | >16 | 1 | A2059G | H69R | WT |
| M6593 | 2008 | Norway | 16 | >16 | 0.5 | 2 | 0.125 | 0.063 | A2059G | WT | WT |
| M6735 | 2010 | Norway | >16 | ≥64 | 0.5 | 16 | 8 | 1 | A2059G | H69R | WT |
| M6712 | 2010 | Australia | >16 | ≥64 | 0.5 | >16 | 8 | 1 | A2059G | H69R | S81N |
| M6711 | 2010 | Australia | >16 | ≥64 | 0.25 | >16 | 8 | 1 | A2059G | WT | WT |
| M6271 (22) | 2004 | Australia | >8 | >32 | 0.5 | 4 | 0.125 | 0.25 | A2058G | WT | WT |
| M6257 (22) | 2004 | Sweden | >8 | > 16 | 2 | 1 | 0.25 | 0.5 | A2058G | WT | WT |
| M6321 (22) | 2004 | Australia | ≥64 | ≥64 | 1 | 2 | 0.03 | 0.125 | A2058G | WT | WT |
| M6604 | 2009 | Denmark | 64 | >16 | 2 | 4 | 0.25 | 0.5 | A2058G | WT | WT |
| M6714 | 2010 | Norway | >16 | ≥64 | 4 | 16 | 4 | 1 | A2058G | WT | WT |
| M6302 (22) | 2004 | Sweden | >8 | >16 | 16 | 8 | 0.25 | 0.125 | A2058C | WT | WT |
| M6848 | 2008 | United States | >16 | ≥64 | 4 | ND | ND | ND | A2058C | WT | WT |
a
Strains without a reference have not been described previously.
b
AZM, azithromycin; ERY, erythromycin; SOL, solithromycin; CIP, ciprofloxacin; MXF, moxifloxacin; DOX, doxycycline; ND, not determined.
c
Macrolide resistance-mediating mutations in the 23S rRNA gene (E. coli numbering) and mutations in the genes encoding the ribosomal proteins L4 and L22 (M. genitalium numbering). WT, wild-type sequence of the gene.
d
M6090, M6151, and M6312 are consecutive isolates from the same patient.