FIG 2.

Fold decrease in mean EC₅₀s in the presence of different percentages of human serum with the in vitro antiviral activity determined using the C8166 LTR luciferase reporter cell line. The HIV-1 NL4.3 virus was used to infect the reporter cell line in RPMI medium containing 10% FBS that was supplemented with 0, 10, 20, 30, 40, or 50% human serum. Data represent the averages from three separate experiments with the standard deviations shown. Extrapolation to 100% human serum gives a 2.14-fold increase in the EC₅₀ for BI 224436.