FIG 2.

HTS for inhibitors of the NoV GII.4 RdRp. Compounds were screened for inhibitory activity against the NoV RdRp. (A) Control measurement for test plates in chronological order. The Z′ factor and Z factor were determined for each plate, and a value of >0.5 was considered acceptable, which is highlighted with a dashed red line. (B) Inhibition results of the HTS. RdRp inhibition was calculated as a percentage of internal plate controls. The hit selection cutoff, which represented inhibition exceeding 3 times the SD of the mean distribution, is shown as a dashed red line.