FIG 5.
Inhibitory profiles of lead NoV inhibitors against RdRps from related caliciviruses. (A) Unrooted neighbor-joining tree of RdRp amino acid sequences used in this study. The evolutionary distances were computed using the number-of-differences method and represent the number substitutions per sequence, indicated by the scale bar. (B) Amino acid percentage identities between the analyzed enzymes. (C) Inhibitory activities of the identified compounds against enzymes from related viruses. The compounds were assayed in triplicate at increasing concentrations, and average changes in IC50s are shown with standard deviations for each enzyme relative to the target, GII.4 Den Haag 2006b RdRp.