FIG 6.
Inhibition of the Norwalk subgenomic replicon (SGR) by lead norovirus NNIs. (A) The inhibitory activities of the top four compounds were assessed using Huh-7 cells harboring the Norwalk replicon (HG23 cells). NIC02 and NIC04 inhibited the replicon with EC50s of 30.1 μM and 71.1 μM, respectively. NIC10 and NIC12 had limited effect on the replication of the replicon. (B) Cytotoxicities of the NIC compounds were examined at concentrations ranging from 1 μM to 100 μM. Only NIC02 demonstrated cytotoxic effect at high concentrations (CC50 = 134 μM). All results are the averages of triplicate experiments plotted with standard deviations.