FIG 3.

gp120-CD4-His tag binding assay. Two representative 2-aminothiazolone derivative molecules, 16h and 16l, were tested in a competitive ELISA to demonstrate that gp120 binding to CD4 was the target of this class of compounds. Both compounds were able to displace soluble CD4 from a dual tropic gp120 in a dose-dependent manner (IC₅₀ for both compounds, 20 μM). DMSO was also tested at the same concentrations present at each compound dilution. The experiments were repeated twice in triplicate.