FIG 6.

Dose-response curves for the P_ywaC promoter-reporter in the absence and presence of osmoprotectants, leading to 9 novel cell wall-active inhibitors. Peak responses of the P_ywaC reporter were measured in the presence (black circles) and absence (gray circles) of osmoprotectants (MSM) for 25 priority active compounds, of which 9 phenocopied known cell wall-active antibiotics, identifying them as cell wall-active compounds. Fold increases in luminescence were calculated by dividing the RLU value in the presence of inhibitor by the value for the 1% DMSO control.