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NADH-dependent reduction of acceptors by TvIsf3. Panels A and B demonstrate that both NADH and a substrate are required to observe the turnover activity of TvIsf3. The anaerobic spectrophotometric assays were performed in an assay buffer with air-saturated water, metronidazole, or chloramphenicol as a substrate and NADH as a reductant. The details are described in Materials and Methods.
