FIG 1.

2D gels of bronchoalveolar lavage fluid (BALF) samples. An aliquot of 50 μg of protein was subjected to 2-dimensional gel electrophoresis using strips of pI 5 to 8 followed by 12.5% SDS-PAGE gel in the second dimension. The marked spots were excised, trypsin digested, and analyzed by MS. Spot 1, haptoglobin (Hp); spots 2 and 3, C-reactive protein (CRP); spot 4, annexin A1 (Anx A1). (A) BALF from infected untreated animal demonstrates abundant presence of both Hp and CRP, whereas Anx A1 is almost undetectable. (B) BALF from treated rabbit is characterized by the absence of Hp and CRP with the presence of Anx A1 (similar to the uninfected control). (C) BALF from uninfected control (healthy rabbit) shows the absence of Hp and CRP, while Anx A1 is present. Gels were stained with Sypro ruby for visualization.