TABLE 2.

Proportion of heteroresistance at resistance-conferring loci in gyrA (Rv0006) and gyrB (Rv0005)^a

Ofloxacin MIC	Study no.	Mutation by Sanger sequencing	Proportion of heteroresistance at:							Total
			Rv0005 site (mutation)		Rv0006 site (mutation)
			1613 T (N538I)	1616 A (T539N)	269 T (A90V)	271 C (S91P)	280 A (D94N)	280T (D94Y)	281 G (D95G)
1	15		0	0	0	0	0	0	0	0
1	16		0	0	0	0	0	0	0	0
1	17		0	0	0	0	0	0	0	0
1	18		0	0	0	0	0	0	0	0
1	19		0	0	0	0	0	0	0	0
1	20		0	0	0	0	0	0	0	0
1	21		0	0	0	0	0	0	0	0
1	22		0	0	0	0	0	0	0	0
1	23		0	0	0	0	0	0	0	0
1	24		0	0	0	0	0	0	0	0
1	25		0	0	0	0	0	0	0	0
4	29		0	0.23b	0.09	0.22	0	0	0.45	0.99
8	2	A90V	0	0	0.84	0	0	0	0	0.84
8	28	D94N	0	0	0	0	1	0	0	1
8	45		0	0	0	0	0	0	0.09	0.09
16	38		0	0	0	0	0	0	0	0
64	1	D94G	0	0	0	0	0	0	1	1
64	6	D94G/D94N	0	0	0	0	0.49	0	0.5	0.99
64	7	D94Y	0	0	0	0	0	1	0	1
64	8	D94G	0	0	0	0	0	0	1	1
64	11	D94G	0	0	0	0	0	0	1	1
64	12	D94G	0	0	0	0	0	0	0.48	0.48
64	13	D94Y	0	0	0	0	0	1	0	1
64	14	S91P	0	0	0	1	0	0	0	1
64	40		0	0	0.01	0	0	0	0	0.01
64	41	D94G	0	0	0	0	0	0	0.37	0.37
64	44	N538I	0.98	0	0	0	0	0	0	0.98
128	9	D94N	0	0	0	0	1	0	0	1
128	27		0	0	0	0	0	0	0	0
128	36		0	0	0	0	0.01	0	0	0.01
256	4	D94G/D94N	0	0	0	0	0.82	0	0.17	0.99
256	5	D94G/D94N	0	0	0	0	0.28	0	0.19	0.47
256	10	D94G	0	0	0	0	0	0	1	1
256	26		0	0	0	0	0	0	0	0
256	35		0	0	0.19	0	0	0	0.01	0.2
256	37		0	0	0	0	0	0	0	0
256	42		0	0	0	0	0	0	0.04	0.04

^aIsolates are sorted by MIC and then by sample number. The proportion of heteroresistance was <1% in samples 36 and 40.

^bHeteroresistant positions are shown in bold type.