FIG 2.

Experimental protocol to assess the in vivo drug activity on M. abscessus infection. (1) ZF embryos were i.v. infected with ≈300 CFU of R-M. abscessus expressing tdTomato and distributed and incubated into 96-well plates. From 1 dpi, embryos were exposed to the drugs of interest, which were directly added to the wells. (2) Drugs were then removed and renewed daily for 5 days. (3) To determinate the in vivo antibacterial effects of the drugs, the embryo survival, the bacterial loads, and the evolution of the infection process were monitored at a spatiotemporal level by videomicroscopy.