Interaction of Hsp90 inhibitor with E. histolytica protein extract in pulldown assay. Denatured proteins for Hsp90 inhibitor (SMDC-304198, 304201, 304203, 304205, 304209, and SNX-2112) pulldowns and E. histolytica extract were run on a SDS-PAGE gel and immunoblotted with polyclonal anti-Plasmodium falciparum Hsp90 antibody. M indicates protein marker; molecular weights (kDa) are shown to the right.