FIG 2.

Killing kinetics of HDP mimetics versus C. albicans GDH2346. (A to C) Kinetics of killing against the yeast form. Compounds were diluted in RPMI-MOPS and added to C. albicans as in IC₅₀ assays. Samples were removed at the indicated time points, serially diluted in YPD medium, and then plated on YPD to determine viable CFU. Each line represents increasing concentrations of the drug as a multiple of the MIC. (A) Compound 2 (519); (B) compound 4; (C) Compound 5. (D) Killing of the hyphal form. C. albicans (GDH2346) was grown in 10% FCS for 3 days to achieve hyphae. Hyphae were treated with compound 2 (8 μg/ml) for 0 min (a), 15 min (b), 30 min (c), or 60 min (d). Cultures were stained with FungaLight Live-Dead stain (Invitrogen) and observed under fluorescence microscopy (magnification, ×100). Green, intact cell membrane; red, damaged membrane.