FIG 4.

In vivo activities of HDP mimetics in mouse models of oral candidiasis. (A) Immunosuppressed mouse model. Mice (n = 5) were immunosuppressed with cortisol acetate, followed by oral infection with C. albicans GDH2346. (B) Steroid-free mouse model. Mice (n = 5) were infected with C. albicans GDH2346 after scoring the tongues as described in Materials and Methods. In both cases, the infection was allowed to proceed for 3 days, followed by a single treatment of 50 μl of each compound in the hydrogel. After 24 h, the mice were sacrificed and the tongues were homogenized. The homogenates were diluted and plated for viable colonies. Kidneys revealed no colonies of Candida albicans with or without treatment. The data are representative of two (A) or three (B) independent experiments. For panel B, a separate group of mice were treated with compound 4 and the tongues were harvested after 72 h (labeled 4, day 3).