FIG 2.

RT-PCR analysis of choline/ethanolamine kinase (CEK) and ethanolamine kinase (EK) gene expression levels in L. donovani. CEK and EK overexpression in L. donovani-transfected promastigotes (T) with respect to control promastigotes (C) was determined by RT-PCR at different cDNA dilutions (1:10 and 1:20). RT-PCR was carried out for 35 cycles and the products were run in 1% agarose gel as described in Materials and Methods. GADPH expression levels were used as loading controls. Data are representative of three independent experiments.