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VGP-106 increases mitochondrial ROS production in L. donovani. Mitochondrial ROS levels were measured using the fluorescent dye Mitosox red after treating the promastigotes with or without 10 and 30 μM VGP-106 for 1 and 3 h at 28°C. Data are means ± SD of three independent experiments. Significant differences were determined using Student's t test (*, P < 0.01 versus control).
