TABLE 2.
Susceptibility to VGP-106 of L. donovani lines overexpressing CEK or EKa
| Plasmid | EC50 (μM) |
|
|---|---|---|
| Promastigotes | Amastigotes | |
| pXG | 0.36 ± 0.09 | 0.45 ± 0.03 |
| pXG-CEK | 0.36 ± 0.09 | 0.42 ± 0.05 |
| pXG-EK | 0.43 ± 0.05 | 0.35 ± 0.03 |
a
Control (pXG) and transfected (pXG-CEK and pXG-EK) parasites were grown as described in Materials and Methods for 72 h at 28°C (promastigotes) or 37°C (intracellular amastigotes) in the presence of increasing concentrations of compound. Data are means ± SD of three independent determinations.