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UV absorbance spectral scans (400 to 500 nm) showing the effect of NADPH and 15 min of incubation on metabolite intermediate complex formation of isoniazid (250 μM) with pooled (n = 10) human liver microsomes (A), recombinant CYP2A6 (B), and recombinant CYP2B6.1 and CYP2B6.6 (C).
