TABLE 1.

In vitro susceptibilities for clinical and mutant isolates included in the study and for quality-control strains, as determined by EUCAST or Etest^a

Isolate type and mutant or strain	EUCAST MIC (mg/liter) (susceptibility classification) forb:					Etest MIC (mg/liter) (susceptibility classification) forc:
	ANI	MICA	VOR	ISA	FLU	CAS	AMB
Clinical isolates or QCd strain
SCL 1130.04e	<0.008 (S)	<0.008 (S)	0.5 (R)	0.06	>16 (R)	0.125 (S)	0.5 (S)
RRCL 110.1f	0.03 (S)	>1 (R)	4 (R)	0.5	>16 (R)	2 (R)	0.5 (S)
ATCC 22019g	0.125 (I)	>1 (I)	<0.03 (S)	<0.03	2 (S)	0.5 (S)	0.38 (S)
Mutant isolates or QC strain
SSWT SC5314h	0.015 (S)	<0.008 (S)	<0.03 (S)	<0.03	0.25 (S)	0.19 (S)	0.25 (S)
RRMH2h	0.06 (R)	0.03 (R)i	<0.03 (S)	<0.03	0.25 (S)	0.75 (R)	0.19 (S)
RSMH1h	0.015 (S)	0.03 (R)j	<0.03 (S)	<0.03	<0.125 (S)	0.25 (S)	0.25 (S)
SRMH1h	0.015 (S)	0.008 (S)	<0.03 (S)	<0.03	0.25 (S)	0.25 (S)	0.25 (S)
RRMHO2h	0.06 (R)	1 (R)k	<0.03 (S)	<0.03	<0.125 (S)	1 (R)	0.38 (S)
ATCC 6258g	0.06 (S)	0.125	0.125 (S)	<0.03	16 (R)	0.38 (I)	0.75 (S)

^aSusceptibility classifications were performed according to the EUCAST breakpoints (BP), except for CAS and Etest, for which CLSI BP were adopted, as recommended by the manufacturer.

^bANI, anidulafungin; MICA, micafungin; VOR, voriconazole; ISA, isavuconazole; FLU, fluconazole; S, susceptible; R, resistant; I, intermediate.

^cCAS, caspofungin; AMB, amphotericin B.

^dQC, quality control.

^eThe clinical isolates 952.04 and 5104.04 revealed the same MICs within ±1 dilution step difference; these strains do not carry coding FKS1 HS1 or HS2 mutations.

^fThe clinical isolate 111.12 revealed the same MIC within ±1 dilution step difference; these strains carry the mutations R647R/G and P649P/L.

^gQuality-control strains.

^hSC5314 is the wild-type parental strain; mutations carried by the strains are as follows: RR_MH2, R647R/G and P649P/L; RS_MH1, R647R/G; SR_MH1, P649P/L; and RR_MHO2, R647G and P649L.

ⁱMicafungin trailing 0.03 to 1 but stays at <50% endpoint in this range.

^jMicafungin creeping increase 0.03 to 0.25.

^kMicafungin trailing 0.06 to 0.25 and just >50% endpoint in this range.