Fig. 8.

Expression of human tau does not robustly rescue defects observed in the ptl-1 null mutant, but phenotypes observed in tau transgenic lines are dependent on endogenous PTL-1. ‘htau Tg’ refers to the human tau transgene, and the terms ‘wild-type’ and ‘ptl-1(ok621)’ immediately following this refer to the genotype at the ptl-1 locus, whether wild-type or ok621 mutant, respectively. (A) Neuron imaging time course of htau transgenic worms showing data for anterior touch receptor neurons visualised using the Pmec-4::gfp reporter. (Ai) Control strains together with the htau Tg; ptl-1(ok621) rescue strain. (Aii) Effect of the htau Tg alone, compared with wild-type controls. Worms were imaged every second day from day 1 to day 15. Sample sizes are indicated below the graph. Control strains, wild-type and ptl-1(ok621), are the same as those shown for the PTL-1 transgenics (Fig. 6A). The χ² test for independence was used to analyse differences between genotypes; ns, no significance; *P<0.05. (B) Survival curves for htau transgenic worms. n = 100 for each strain at the start of the assay. Results of statistical analysis are shown in the table below. Control strains are the same as those shown for the PTL-1 transgenics (Fig. 6B).